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Abstract:
To observe hypoglycemic and hypolipidemic effects of
Herba Agrimoniae ethanol-extract in T2DM Rats and data
analysis of its dose-response relationship. Except 10
SPF SD rats were as the control group, the rest 60 T2DM
Rats were induced by feeding with high-fat and
high-sugar diet and intraperitoneal injection of
low-dose streptozotocin (STZ, 35mg/kg). According to the
values of blood glucose concentration (≧11.1mmol/L), the
rats were randomly divided into diabetic model group,
three Herba Agrimoniae ethanol-extract groups of high
dose (40g•kg-1), medium dose (20g•kg-1) and low dose
(10g•kg-1), and positive control group (glibenclamide,
0.30mg/kg). During 36 d of continuous intragastric (ig)
administration, body weights of the rats were measured
weekly, the values of fasting blood glucose (FBG)
concentration were measured on 7, 14, 21, 28, 35 d after
administration. After the last administration, fasting
10h, then, the rats were decapitated after anesthesia.
Blood of the rats were collected for the analysis of
serum insulin (INS), total cholesterol (TC),
triglycerides (TG) and low density lipoprotein (LDL-C)
levels. Compared with the control group, FBG of model
group rats were significantly increased (P<0.01). After
administration 21d, compared with the model group, FBG
of Herba Agrimoniae ethanol-extract groups of high dose
and medium dose were significantly lower(P<0.01 or
0.05); after administration 28d, compared with the model
group, FBG of Herba Agrimoniae ethanol-extract groups of
high dose, medium dose, low dose and positive control
group were significantly lower (P<0.01 or 0.05).
Compared with the control group, INS levels of model
group were significantly lower (P<0.01); compared with
the model group, INS levels of Herba Agrimoniae
ethanol-extract medium dose group were significantly
increased (P<0.05). Compared with the control group, TC,
TG and LDL-C levels of model group rats were
significantly increased (P<0.01), TG of positive control
group was significantly increased (P<0.05). Compared
with the model group, TC and LDL-C levels of Herba
Agrimoniae ethanol-extract medium dose group were
significantly lower (P<0.05) and TG levels was
significantly lower (P<0.01); TG levels of positive
control group was significantly lower (P<0.01). Herba
Agrimoniae ethanol-extract can decrease blood glucose,
regulate blood lipids in T2DM Rats. Among them, the
medium dose group had the best effect. |
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